P001 - Knock-In Allelic Series

In order to gain a deeper understanding of the pathophysiological mechanisms in Huntington’s disease (HD), we compared the proteomes between wild-type and heterozygous Huntingtin knock-in mice with increasing CAG repeat lengths in a number of different brain regions and peripheral tissues at three different ages (2, 6, and 10 months). The analysis of more than 1,200 tissue samples with on average 8,000 quantified proteins comprises one of the largest global, quantitative proteomics studies published so far. More importantly, it allows a systematic analysis of pathways and interaction networks on the protein level, the identification of novel target candidates, and provides a comprehensive resource for training of system biology models. Part of the data is described in the paper

Peter Langfelder, Jeffrey P Cantle, Doxa Chatzopoulou, Nan Wang, Fuying Gao, Ismael Al-Ramahi, Xiao-Hong Lu, Eliana Marisa Ramos, Karla El-Zein, Yining Zhao, Sandeep Deverasetty, Andreas Tebbe, Christoph Schaab, Daniel J Lavery, David Howland, Seung Kwak, Juan Botas, Jeffrey S Aaronson, Jim Rosinski, Giovanni Coppola, Steve Horvath, X William Yang: "Integrated genomics and proteomics define huntingtin CAG length-dependent networks in mice", Nature Neuroscience 19 (2016) 622-633. PMID: 26900923,